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D-阿洛酮糖对载脂蛋白A-I基因的胰岛素的模仿作用

Insulin mimetic effect of D-allulose on apolipoprotein A-I gene

Michael J. Haas,Shrina Parekh,Poonam Kalidas,Angela Richter,Firas Warda,Norman C. W. Wong,Masaaki Tokuda,Arshag D. Mooradian,et al.

First published: 05 January 2022

https://doi.org/10.1111/jfbc.14064

Abstract: Several nutrients modulate the transcriptional activity of the apolipoprotein A-I (apo A-I) gene. To determine the influence ofrare sugars on apo A-I expression in hepatic (HepG2) and intestinal derived (Caco-2) cell lines, apo A-I, albumin, and SP1were quantified with enzyme immunoassay and Western blots while mRNA levels were quantified with real-time polymerasechain reaction. The promoter activity was measured using transient transfection assays with plasmids containing varioussegments and mutations in the promoter. D-allulose and D-tagatose, increased apo A-I concentration in culture media whileD-sorbose and D-allose did not have any measurable effects. D-allulose did not increase apo A-I levels in Caco-2 cells.Thesechanges paralleled the increased mRNA levels and promoter activity. D-allulose-response was mapped at the insulinresponse core element (IRCE). Mutation of the IRCE decreased the ability of D-allulose and insulin to activate the promoter.Treatment of HepG2 cells, but not Caco-2 cells, with D-alluose and insulin increased SP1 expression relative to control cells.D-allulose augmented the expression and IRCE binding of SP1, an essential transcription factor for the insulin on apo A-Ipromoter activity. D-allulose can modulate some insulin-responsive genes and may have anti-atherogenic properties, in partdue to increasing apo A-I production.

 

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